Figure 6: EMT-like cells are not proliferative. | Nature Communications

Figure 6: EMT-like cells are not proliferative.

From: Concomitant Notch activation and p53 deletion trigger epithelial-to-mesenchymal transition and metastasis in mouse gut

Figure 6

(a) Immunohistochemical staining of P21 in NICD/p53−/− primary tumours, showing nuclear P21 expression in cancer cells at the tumour front. This feature is emphasized in cancer cells invading the desmoplastic area. Scale bars, 100 μm. (b) Triple immunofluorescence on NICD/p53−/− primary tumours using 4′,6-diamidino-2-phenylindole (grey), GFP staining (green), ECAD (blue) and phospho-H3 (red). Scale bars, 40 μm. (c–e) Magnification of different tumour cell features coming from the same slide showing some epithelial cells in clusters (c) or fibroblasts (e) in division whether EMT-like cells are quiescent (d). Scale bars, 4 μm. (f) Proportion of pH3-positive cells among GFP-positive cells. Among GFP-positive cells, 12% of cells with an epithelial morphology as defined by ECAD positivity (E+; n=585) are proliferative, while only 2% of ECAD-negative cells (E−; n=274) express pH3 (full contingency table indicates dependency χ2 test, P<0.0001, **). This indicates that cells undergoing EMT-like are less proliferative.

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