Figure 5: Total protein accumulation and Km values of the NC99-like NA proteins with different amino acid substitutions. | Nature Communications

Figure 5: Total protein accumulation and Km values of the NC99-like NA proteins with different amino acid substitutions.

From: Epistatic interactions between neuraminidase mutations facilitated the emergence of the oseltamivir-resistant H1N1 influenza viruses

Figure 5

(a) Representative western blotting showing total accumulation of the NC99-like NA proteins (NAGA/17and NAGA/20 (H275Y)) with the indicated single substitutions; the variant NAs were expressed by equal quantities (0.5 μg) of the respective plasmids. Black and grey indicate wt and H275Y-mutant NA proteins, respectively. (b) NA Km values of rgBR/59/07 viruses carrying NAGA/17 or NAGA/20 (H275Y) with the indicated single substitutions. Dotted and dashed lines indicate Km values of NAGA/17 and NAGA/20 (H275Y), respectively. (c) Representative western blotting showing total accumulation of NAGA/17 and NAGA/20 (H275Y) proteins with sequentially added substitutions (sub), the order is the same in next panel; NAs were expressed by equal quantities (0.5 μg) of respective plasmids. (d) NA Km values of rgBR/59/07 viruses carrying the NAGA/17 and NAGA/20 (H275Y) with sequentially added substitutions. Dotted and dashed lines indicate Km values of NAGA/17 and NAGA/20 (H275Y), respectively. All graphs show mean±s.e.m. of three to five times independent experiments. *P<0.05, two-tailed t-test, versus NAGA/17; †P<0.05, two-tailed t-test, versus NAGA/20 (H275Y).

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