Figure 4: Visual functions of cortical PV cells in adult PV-Cre;Ai9 and PV-Mecp2−/y;Ai9 mice. | Nature Communications

Figure 4: Visual functions of cortical PV cells in adult PV-Cre;Ai9 and PV-Mecp2−/y;Ai9 mice.

From: Conditional deletion of Mecp2 in parvalbumin-expressing GABAergic cells results in the absence of critical period plasticity

Figure 4

(a) Top: Two-photon laser imaging of a tdTomato-expressing cortical PV cell (red) and an example trace of extracellular spike. The recording micropipette was filled with Alex fluor-488 (green). Scale bar, 20 μm. Bottom: Spikes evoked by drifting gratings from the recorded PV cell. (b) Receptive fields of an example V1 PV cell recorded from adult PV-Cre;Ai9 (left) and PV-Mecp2−/y;Ai9 mice (right). (c) Polar plots of spike response preferences of all examined cortical PV cells in adult PV-Cre;Ai9 (red, n=26 cells from 7 mice, top) and PV-Mecp2−/y;Ai9 mice (green, n=19 cells from 4 mice, bottom). (d) The magnitudes of averaged baseline spontaneous (left) and evoked spike activity (right) of cortical PV cells from the experiments in c. Each dot represents data from a cell, and bars are mean±s.e.m. The t-test was used. (e) The dependence of spike rate on the spatial (left) or temporal (right) frequency of drifting gratings at the optimal orientation of adult cortical PV cells. No significant difference was found between the two genotypes.

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