Table 1 Membrane properties of layer 4 PV and PCs from four mouse lines at different postnatal days.

From: Conditional deletion of Mecp2 in parvalbumin-expressing GABAergic cells results in the absence of critical period plasticity

Postnatal day

P30

 

P50

PV cell

WT

n

PV-Cre:Ai9

n

Flox-Mecp2

n

PV-Mecp2 −/ y

n

PV-Mecp2 −/y

n #

Membrane resistance (MΩ)

57.77±4.52

20

59.85±2.84

44

49.90±4.79

17

80.88±6.23

35*

101.83±6.90

21*

Membrane capacitance (pF)

60.66±2.51

20

63.09±2.37

44

70.75±5.55

17

55.17±2.29

35

45.04±2.76

21**

Resting potential (mV)

−69.29±0.83

13

−68.26±0.55

25

−69.33±0.48

8

−67.50±0.59

22

−68.67±0.55

19

Spike threshold (mV)

−30.43±0.81

13

−29.55±0.76

25

−30.74±0.98

8

−33.20±0.79

22*

−32.45±1.24

19

Spike half-width (ms)

0.24±0.01

13

0.23±0.01

25

0.20±0.01

8

0.25±0.01

22

0.28±0.01

19

AHP amplitude† (mV)

−15.40±1.13

13

−20.59±0.60

25**

−16.69±1.18

8

−19.40±0.57

22**

−21.95±0.97

19*

Rheobase‡ (pA)

547.7±60.1

13

448.0±38.2

25

537.5±83.3

8

335.2±32.9

22**

265.8±13.8

19

I/O slope§ (Hz pA−1)

0.30±0.02

13

0.45±0.03

25**

0.36±0.05

8

0.41±0.03

22**

0.47±0.03

19*

Pyramidal cell

WT

n

PV-Cre:Ai9

n

Flox-Mecp2

n

PV-Mecp2 −/y

n

  

Membrane resistance (MΩ)

130.72±5.30

36

118.66±8.18

29

122.2±7.38

33

114.39±7.67

17

  

Membrane capacitance (pF)

93.56±3.67

36

95.02±3.33

29

−85.78±4.83

33

92.26±5.80

17

  

Resting potential (mV)

−72.87±0.77

13

−72.54±1.22

17

−73.53±0.97

8

−74.63±1.61

10

  

Spike threshold (mV)

−34.96±101

13

−36.91±0.96

17

−35.09±1.14

8

−36.53±0.46

10

  

Spike half-width (ms)

0.52±0.02

13

0.77±0.01

17**

0.58±0.02

8

0.79±0.03

10**

  

AHP amplitude† (mV)

−15.84±0.96

13

−13.28±1.08

17

−15.71±1.61

8

−13.50±0.59

10

  

Rheobase‡ (pA)

81.53±5.29

13

92.50±4.79

17

82.5±13.9

8

96.00±6.53

10

  

I/O slope§ (Hz pA−1)

0.13±0.01

13

0.12±0.01

17

0.12±0.01

8

0.10±0.01

10*

  
  1. PC, pyramidal cell; PV, parvalbumin-expressing cell.
  2. Step currents with duration of 600 ms and a range up to 1 nA were used to trigger action potentials (see Fig. 5). Data are presented as mean±s.e.m. and the t-test was performed for statistical comparison. *0.01<P<0.05; **P<0.01.
  3. #The statistic analysis of data difference was made versus P30 PV-Mecp2−/y.
  4. †AHP was calculated as the voltage difference between the action potential threshold and the most negative level reached during the repolarizing phase.
  5. ‡Rheobase was the magnitude of the first current step that evoked action potentials.
  6. §The I/O slope was the slope of output (firing frequency) versus input (injected current) curve, with injection currents ranging above the rheobase.
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