Figure 2: SpnD39III restriction.
From: A random six-phase switch regulates pneumococcal virulence via global epigenetic changes
(a–h) Restriction by SpnD39III was demonstrated by transformation of differently methylated pDP28 plasmids into strains expressing only one SpnD39III variant. Restriction was evident in strains expressing variant SpnD39IIIB (b) and SpnD39IIIC (c), which have non-co-directional target sites on pDP28 (Supplementary Fig. 4), whereas restriction was not evident in strains expressing variants SpnD39IIIA (a) or SpnD39IIID (d), which have co-directional target sites on pDP28. Graphs indicate s.d. of three independent replicates. Statistically significant differences found using an analysis of variance Tukey test are shown. When utilizing recombinant pDP28 derivatives with two non-co-directional target sites for SpnD39IIIA (e) or SpnD39IIID (h) restriction could also be observed for these sites, whereas no restriction was observed when transforming a recombinant pDP28 with only one SpnD39IIIB site (f) or two co-directional SpnD39IIIC sites (g). ***P<0.001.
