Figure 3: In vitro phenotypes of SpnD39IIIA-F variant strains.
From: A random six-phase switch regulates pneumococcal virulence via global epigenetic changes
(a) Phagocytosis of pneumococci by RAW 264.7 macrophages. Mean values of three independent replicates and s.d. are shown. (b) LuxS expression was assayed by quantitative western blot and the mean of quadruplicate samples are shown (in case of SpnD39E-F including two technical replicates). (c) The production of the type 2 polysaccharide capsule was quantified using an uronic acid assay and the mean and s.d. of quadruplicate samples are shown (in case of SpnD39E-F including two technical replicates). Statistical analysis of in vitro experiments (a–c) was performed using one-way analysis of variance and a Tukey post-comparison test. *P<0.05, **P<0.01, ***P<0.001.
