Figure 5: Regulatory T cells suppress GM-CSF production by deprivation of IL-2.
From: Multiple sclerosis-associated IL2RA polymorphism controls GM-CSF production in human TH cells
(a,b) Sorted naive (TN) and regulatory T cells (Treg) were co-cultured in a 1:1 ratio and activated (αCD3/αCD28) and the indicated blocking antibodies for 5 d (n=9,9,4,3, respectively). Shown are representative examples of GM-CSF secretion and carboxyfluorescein succinimidyl ester (CFSE) dilution (a) and cumulative results of GM-CSF secretion (b). Data are combined from three independent experiments. (c) Fluorescent imaging of TN (CFSE labelled) and Treg cells (CellTrace Violet labelled), which were stimulated together with 10 ng ml−1 IL-2Fc for 10 min. Each symbol represents a single individual. For all figures error bars show mean±s.e.m.
