Figure 5: DCs expressing C/EBPβ antagonize apoptosis in lymphoma cells.

(a) Eμ-Myc tumour cells were co-cultured with Wt BM myeloid DCs or C/EBPβ^−/− DCs (B6). Lymphoma cell survival was determined as in Supplementary Fig. 5a,b (n=5 individual Eμ-Myc cell clones tested). Data are presented as fold change in lymphoma cell survival in the presence of DCs versus absence of DCs. Bars represent means±s.e.m.; a Student’s t-test was used. *P<0.05; **P<0.01. (b) C/EBPβ^−/− mice and Wt or heterozygous littermates as controls were challenged with 1 × 10⁴ Eμ-Myc GFP cells i.v. Tumour load in spleens of C/EBPβ^−/− (n=5) compared with controls (n=6) was assessed with flow cytometry on day 7 (n=2 independent experiments). (c–f) Culture supernatants were obtained from co-cultures as in a, followed by determination of cytokine content in cytokine bead arrays. In b–f, bars represent means±s.e.m.; a Mann–Whitney test was applied. *P<0.05; **P<0.01; ***P<0.001.