Figure 7: Lymphoma-derived factors control C/EBPβ isoform ratios.

DCs from naive and tumour-challenged mice (days 7–10) were isolated as in Fig. 3a, and protein lysates were analysed using SDS–PAGE and immunoblotting. (a) All three isoforms of C/EBPβ were detected with anti-C/EBPβ antibody, and anti-GAPDH was used as loading control. Images have been cropped for presentation; a full size image is presented in Supplementary Fig. 8. (b) The ratios of LAP/LAP* to LIP were determined by densitometric scanning and are expressed as bar graphs (n=4 independent experiments, with >n=20 mice per group). Bars represent means±s.e.m., a Mann–Whitney test was applied. (c–f) Myeloid DCs from Wt, LIP and LAP knock-in mice were generated and co-cultured as in Fig. 5a with Eμ-Myc lymphoma B cells, and culture supernatants were analysed by cytokine bead array or ELISA in Fig. 5c–f. Graphs depict means. *P<0.05; **P<0.01; ***P<0.001. A Mann–Whitney test was applied.