Figure 2: Association of chromatids in meiosis II of R. pubera.
From: Chiasmatic and achiasmatic inverted meiosis of plants with holocentric chromosomes
(a) Different classes of cells with individualized chromatids in prophase II. Class I comprises cells with eight or more individualized chromatids, Class II cells have four or six, class III have two and class IV represents cells without individualized chromatids. Daughter cells exemplifying each class are highlighted. (b) Frequency of the different classes of cells with isolated chromatids during prophase II (n=55). (c) Pairs of chromatids of prophase II cells connected with a thin DAPI (blue)-stained thread or in close association. CMA staining (yellow) indicates regions of 45S rDNA. (d) Quantification of chromosome mis-segregation in meiosis I and II (n=37 and 36 cells, respectively). Size bars correspond to 10 μm.
