Figure 3: Detection of the target HIV-1 gag RNA using the PNA-SP4/PNA-RP25 detection system.
From: Quantification of plasma HIV RNA using chemically engineered peptide nucleic acids
(a) Structure of PNA-SP4. (b) Structure of PNA-RP25. (c) Time-dependent absorbance (652 nm) changes upon analyzing different concentrations of HIV-1 gag RNA. (d) Signal response plots (absorbance at 450 nm versus concentration of HIV-1 gag RNA) obtained after quenching of enzymatic oxidation with H2SO4. The green line marks the minimal distinguishable differential concentration and represents the lowest limit of quantification. For c and d, values represent the mean±s.d. of duplicate measurement (n=2) for each sample. (e,f) Photographs of the 96-well plate show the variation in colour intensity for HIV-1 gag RNA detection before (e) and after (f) quenching of enzymatic oxidation with H2SO4.
