Figure 1: FLC is a suppressor of thermal induction of flowering. | Nature Communications

Figure 1: FLC is a suppressor of thermal induction of flowering.

From: Jumonji demethylases moderate precocious flowering at elevated temperature via regulation of FLC in Arabidopsis

Figure 1

(a) flc-3 flowers at a similar time as WT when grown in LD 22 °C, but flowering is accelerated in flc-3 when grown under LD 29 °C conditions. Representative 30-day-old plants (LD 22 °C) or 23-day-old plants (LD 29 °C) are shown for comparison. Scale bar, 1 cm. (b) Total primary rosette leaves before bolting in WT and flc-3 plants grown under LD 22 °C and 29 °C conditions were counted (two independent experiments with 15–20 plants each). Numbers below graph indicate leaf number ratio (29 °C/22 °C). Bars indicate standard deviation (s.d.); two-tailed Student’s t-test, **P<0.01. (c) FLC mRNA levels in 5, 9 and 13 DAG seedlings grown under LD 22 °C and 29 °C conditions. Relative fold changes to 5 DAG seedlings grown under 22 °C conditions are presented. Bars indicate s.d. of three biological replicates; two-tailed Student’s t-test, *P<0.05, **P<0.01. (d) Schematic drawing of the FLC genome structure showing regions amplified by primers used for ChIP analysis. Scale bar, 1 kb. (e) ChIP analysis of H3K27me3 enrichment at the FLC locus in 8 DAG WT seedlings grown under LD 22 °C and 29 °C conditions. An anti-H3 antibody was used as a control. Bar indicates standard error of the mean (s.e.m.) of three biological replicates; two-tailed Student’s t-test, **P<0.01.

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