Figure 2: IEC apoptosis and hyperproliferation in A20IEC/myel-KO mice.
From: A20 controls intestinal homeostasis through cell-specific activities
(a) Transmission electron micrographs of small intestinal crypt region of wild-type (WT) and A20IEC/myel-KO mice. Arrows indicate condensed phagocytosed dead cell remnants (Scale bars, 4 μm) (S=stem cell, P=Paneth cell). (b,c) Representative TUNEL staining (b) and immunostaining of active caspase-3 (c) in crypts of the terminal ileum and colon of wild-type (WT), A20IEC-KO, A20myel-KO and A20IEC/myel-KO mice (Scale bars, 50 μm). (d) Quantification of cleaved caspase-3 positive cells in crypts of wild-type (WT, n=5), A20IEC-KO (n=5), A20myel-KO (n=5) and A20IEC/myel-KO mice (n=5). Error bars represent s.e.m. ***P<0.001 using Student’s t-test. (e) Ki67 immunostaining on wild-type (WT) and A20IEC/myel-KO ileal crypts (Scale bars, 5 μm). (f) In situ hybridization for Olfm4 on wild-type (WT) and A20IEC/myel-KO ileal crypts using an Olfm4 specific probe (Scale bars, 5 μm).
