Figure 4: Severe colitis in aged A20IEC/myel-KO mice.
From: A20 controls intestinal homeostasis through cell-specific activities
(a) representative images of AB-PAS stainings of colon sections from 30-week-old wild-type (WT) and A20IEC/myel-KO mice (Scale bars, 50 μm). (b–d) Immunostaining on colonic sections of wild-type (WT) and A20IEC/myel-KO mice for proliferating cells with Ki67 (b), infiltrating leukocytes with CD45 (c) and apoptotic cells with cleaved caspase-3 (d) antibodies (Scale bars, b: 50 μm, c: 20 μm, d: 50 μm). (e) Immunofluorescent staining for Muc-2 and 16S bacterial FISH on colonic sections from 30-week-old wild-type (WT) and A20IEC/myel-KO mice (Scale bars, 50 μm). (f) Representative endoscopic pictures of aged (>40 weeks) wild-type (WT), A20IEC-KO, A20myel-KO and A20IEC/myel-KO mice. (g) Colon inflammation detected by endoscopy is represented as MEICS score in aged (>40 weeks) wild-type (WT, n=5), A20IEC-KO (n=6), A20myel-KO (n=5) and A20IEC/myel-KO (n=8) mice. (h) Histological colitis scoring on aged wild-type (WT, n=8), A20IEC-KO (n=8), A20myel-KO (n=8) and A20IEC/myel-KO (n=8) mice showing strong colitis only in A20IEC/myel-KO mice. Error bars represent s.e.m. ***P<0.001 using Student’s t-test.
