Figure 3: JB253 confers photoswitching on KATP channels.
From: Optical control of insulin release using a photoswitchable sulfonylurea
(a) Photocurrents recorded from HEK293t cells transfected with plasmids encoding Kir6.2 and SUR1 using the whole-cell patch-clamp configuration (holding potential −60 mV). JB253-treated cells respond to wavelengths between 400 and 500 nm with a reduction in the magnitude of the K+ inward rectifier current. (b) JB253 allows reversible and repeated closure of KATP channels in response to light–dark cycles using λ=400 nm to induce cis-isomerization (purple) and relaxation in the dark (black). (c) Kinetics of light-triggered block and thermal restoration of KATP channel currents mediated by JB253. In all cases, traces represent n=4 cells.
