Figure 4: JB253 allows optical manipulation of pancreatic beta cell activity.
From: Optical control of insulin release using a photoswitchable sulfonylurea
(a) Beta cells residing within JB253-treated islets display large increases in cytosolic Ca2+ following exposure to 405 nm (purple) (scale bar, 110 μm) (linear look up table (LUT)). (b) Illumination with 405 nm induces large and synchronous rises in Ca2+ as indicated by Fluo-2 (representative trace from n=10 recordings). (c) JB253 can be used to impose complex dynamics including Ca2+ oscillations (representative trace from n=5 recordings). (d) High-dose tolbutamide (Tb) augments the effects of JB253 on KATP channel blockade (representative traces from n=6 recordings). (e) Diazoxide (Dz) inhibits JB253 effects by opening the KATP channel ion pore (representative traces from n=4 recordings).
