Figure 6: Manipulation of human islet activity using JB253.
From: Optical control of insulin release using a photoswitchable sulfonylurea
(a) JB253-treated beta cells residing within intact human islets respond to 440 nm with rapid rises in cytosolic Ca2+ levels (representative trace from n=8 recordings). (b) Almost 62% of X-Rhod1-loaded cells respond to JB253 with Ca2+ rises. (c) As in a, but imposition of oscillations to demonstrate reversibility of JB253 effects in human tissue (Tb, tolbutamide; positive control) (representative trace from n=4 recordings). (d) Reversal of JB253 action using diazoxide to open the KATP channel ion pore (Dz, diazoxide; negative control) (representative trace from n=5 recordings). In all cases, islets were derived from three donors.
