Figure 1: STEN is essential for directed migration.
From: Evolutionarily conserved coupling of adaptive and excitable networks mediates eukaryotic chemotaxis
(a) LimE-RFP and RBD-GFP were co-expressed in plaA– or plaA–/piaA– Dictyostelium cells treated with 50 μM LY. The cells were stimulated with 1 μM cAMP at 0 s. Left: representative images from time-lapse videos captured at 3 s per frame. Right: quantification of membrane to cytosol ratio of RBD and LimE (mean±s.d. of n=3 cells each) over time. Scale bar, 10 μm. (b) Chemotaxis of cells developed for 5 h treated with 50 μM LY or control volume of DMSO. Scale bar, 20 μm. Cell migration towards the tip of a micropipette filled with 1 μM cAMP (red stars) was recorded at 30 s intervals. Five representative cells in each movie were outlined and traced every 5 frames. (c) Cells were treated with 5 μM latrunculin. A micropipette filled with 1 μM cAMP was placed next to the cell at 20 s and switched to the opposite side of the cell several times. Top: representative images showing the direction of RBD-GFP patches in cells. White dots indicate the location of the micropipette tip. Scale bar, 5 μm. Bottom: kymograph showing the activity of RBD-GFP. Dotted lines indicate direction of the gradient.
