Figure 2: Hypoxia-mediated downregulation in Drosha and Dicer results in decreased miRNA levels.
From: Hypoxia-mediated downregulation of miRNA biogenesis promotes tumour progression
(a) Heat map showing mature miRNA levels under hypoxic conditions, assessed using miRNA array data. (b) Heat map showing precursor miRNA levels under hypoxic conditions, along with the corresponding mature sense (mature) and antisense (mature*) levels. (c,d) Pri-miRNA and mature miRNA expression levels of significantly altered miRNAs under hypoxia exposure in A2780 cells. (e) Pri-miRNA levels in RNA extracted from nuclear fractionated A2780 cells treated with normoxia and hypoxia. (f,g) Precursor and mature miRNA levels in RNA extracted from cytoplasmic fraction of A2780 cells treated with normoxia and hypoxia. Data are presented as mean±s.e.m. of n≥3 experimental groups. *P<0.05, **P<0.01, ***P<0.001 (Student t-test).
