Figure 1: Activation of D3 nanoprobes after apoptosis induction in various cells.
From: An authentic imaging probe to track cell fate from beginning to end
(a) Schematic diagram of D3. (b) MDA-MB231 cells incubated with D3 or Annexin V-cy3 were imaged without or (c) with apoptosis induction using SSP (4 μM) for 4 h. Fluorescence signals were assessed using fluorescence microscopy (magnification: × 40). Scale bar, 100 μm. (d) D3 was incubated with or without apoptosis induction in HeLa and MDA-MB231 cells, and FITC fluorescence intensity in cell lysates was examined. The fluorescence intensity of HeLa cells without treatment was set as onefold. Each experiment was done in triplicate and the data were shown as mean fluorescence±s.d. (e) Free PDL–FITC or the collected cell lysate from D3-labelled and SSP-treated cells was separated by size-exclusion chromatography. The FITC fluorescence intensity of each fraction was plotted. Note that the broad fluorescence peak at later time points indicated low-molecular-weight degraded fragments of PDL.
