Figure 4: Live- and dead-MSC imaging with DL2 in vitro and in vivo.
From: An authentic imaging probe to track cell fate from beginning to end
(a) After incubation hMSCs for 12 h with DL2, fluorescence signals were assessed by fluorescence microscopy for 4 days. Scale bar, 100 μm. (b) After 4 days, hMSCs were incubated with 4 μM of SSP for 4 h to induce cell apoptosis. Fluorescence signals mediated by DL2 were assessed in each FITC and cy5.5 channels. Scale bar, 100 μm. (c) MSCs labelled with DL2 could be visualized in vivo after local injection in the dermis of the mouse ear pinna. MSCs (1~7) labelled with DL2 were strongly fluorescent in the cy5.5 channel. A small portion of the cells (1 and 2) were co-stained in the FITC channel, indicating that they were undergoing cell death. The signal-to-background ratio was 1.20 and 1.35 for cell 1 and cell 2, respectively. Scale bar, 50 μm.
