Figure 2: Transcription induction promotes telomeric aberrations and RNA–DNA hybrids in ALT cells.
From: RNaseH1 regulates TERRA-telomeric DNA hybrids and telomere maintenance in ALT tumour cells
TiTEL transcription was induced for 48 h before performing FISH and CO-FISH experiments. (a) Examples of normal and aberrant tiTELs (arrows) detected by FISH. TiTEL chromosome ends were visualized with lacO PNA probes (green), telomeric repeats with telomeric C-rich probes (red), DNA was counterstained with DAPI (blue). (b) Quantification of aberrations at tiTELs and telomeres at opposite chromosome ends (opp). Untreated samples were set to 1. Bars and error bars are averages and s.d. from at least three experiments where a total of at least 180 tiTEls and opposite chromosome ends were scored. P-values were computed using the Student’s t-test. *P<0.05 (+dox versus −dox). (c) Quantification of telomeric hybrids at tiTELs in U2OS and HeLa cells measured by DIP using the S9.6 antibody. U2OS untreated samples were set to 1. Bars and error bars are averages and s.d. from at least three experiments. P-values were computed using the Student’s t-test. *P<0.05 (+dox versus −dox).
