Figure 2: HyPerRed responds to external H2O2 in mammalian cells.
From: Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide
(a) Images of HyPerRed in transiently transfected HeLa Kyoto cells exposed to 150 μM of H2O2 at 60 s between the first and second picture. Numbers indicate timing in seconds. Scale bar, 40 μm. Lookup table indicates HyPerRed brightness change. (b) Timing of H2O2 induced fluorescence change in the cells expressing HyPerRed shown on panel a. Arrow indicates a time point of H2O2 addition (1 min). (c) Increase in HyPerRed brightness in HeLa Kyoto cells as a function of extracellular H2O2 concentration (values±s.d., 28 cells from three experiments). The inset shows the expanded area of the graph between zero and 30 μM H2O2.
