Table 1 Effect of various oxidants on HyPer.
From: Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide
Oxidant | Source | Fold change in HyPerRed fluorescence±s.d., n=3 | Fold change in HyPer ratio±s.d., n=3 |
|---|---|---|---|
H2O2 | H2O2 (30 nM) | 1.3±0.12 | 1.3±0.12 |
H2O2 | H2O2 (300 nM) | 2.0±0.34 | 3.0±0.32 |
H2O2 | Sodium peroxoborate (300 nM) | 1.8±0.15 | 2.4±0.17 |
Superoxide anion radical | Xanthine (30 μM)–xanthine oxidase (25 mU) | 1.0±0.06 | 1.0±0.05 |
GSSG | Oxidized glutathione GSSG (1 mM) | 1.0±0.03 | 1.0±0.04 |
NO | MAHMA NONOate (1 mM) | 1.0±0.02 | 1.0±0.03 |
ONOO− | 3-morpholinosydnonimine (SIN-1; up to 0.5 mM) | 1.0±0.03 | 1.0±0.05 |
