Figure 8: EGF-dependent signalling requires KIAA1199 in breast cancer-derived cells.
From: NF-κB-induced KIAA1199 promotes survival through EGFR signalling
(a,b) Impaired expression of ErbB members and defective EGFR signalling on KIAA1199 deficiency in breast cancer-derived cells. On the left, serum-starved control or KIAA1199-deficient MCF7 (a) or SK-BR-3 (b) cells were untreated or stimulated with EGF (100 ng ml−1) for the indicated periods of time and the resulting cell extracts were subjected to WB analyses using the indicated antibodies. On the right, total RNAs from control or KIAA1199-depleted (shRNA KIAA1199#2) CaSki cells were subjected to real-time PCR to assess KIAA1199 mRNA levels. The abundance of transcripts in control cells was set to 1 and their levels in KIAA1199-depleted cells were relative to that after normalization with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (c) KIAA1199 promotes EGF-dependent signalling in immortalized breast epithelial cells. Serum-starved control or KIAA1199-depleted NMuMG cells were serum starved and were untreated or stimulated with EGF (100 ng ml−1) for the indicated periods of time. WB analyses were conducted with the resulting cell extracts.
