Figure 3: Binding properties of affinity-matured Alphabodies against human IL-23.
From: Structural basis of IL-23 antagonism by an Alphabody protein scaffold
(a) Comparison of ELISA binding profiles for matured Alphabodies from each maturation campaign against non-matured Cl59 Alphabody. (b) Comparison of KI values obtained via the splenocyte functional assay and KD values determined by equilibrium ELISA. Cl59, MA12 and MB23 are indicated. The thick diagonal straight line corresponds to KI=KD, thin straight lines correspond to KI deviating by a factor 3 from binding KD. Dashed lines represent deviations by a factor 10. (c) Specificity of MA12 and MB23 towards human IL-23 versus human IL-12 determined by ELISA binding assays.
