Figure 2: LRRK2 Roc-COR domain mutants form TSA-sensitive filamentous structures. | Nature Communications

Figure 2: LRRK2 Roc-COR domain mutants form TSA-sensitive filamentous structures.

From: Increasing microtubule acetylation rescues axonal transport and locomotor deficits caused by LRRK2 Roc-COR domain mutations

Figure 2

CV1 (a) or HEK293 (c) cells were transfected with acGFP1-tagged LRRK2 variants (green) as indicated. Twenty-four hours after transfection, the cells were treated with vehicle (Ctrl) or 0.5 μM TSA for 4 h. After treatment, the cells were fixed and processed for immunostaining using anti-acetylated α-tubulin antibody (red). Scale bar, 20 μm (a), 10 μm (c); zoom, 5 μm. The percentage of vehicle (Ctrl) and TSA-treated (TSA) CV1 (b) and HEK293 (d) cells exhibiting filamentous LRRK2 was quantified (mean±s.e.m., zero values are indicated (0)). **P<0.01, ***P<0.001, ****P<0.0001, two-way analysis of variance followed by Fisher’s least significant difference post hoc test. (b) N (coverslips counted, ~50 cells per coverslip; from four experiments)=WT, 7; WT+TSA, 5; Y1699C, 4; Y1699C+TSA, 5; R1441C, 11; R1441C+TSA, 4. (d) N (coverslips counted, ~50 cells per coverslip; from four experiments)=WT, 5; WT+TSA, 5; Y1699C, 13; Y1699C+TSA, 5; R1441C, 15; R1441C+TSA, 5.

Back to article page