Figure 6: Axonal transport and locomotion is restored by TSA treatment.

(a) Kymographs show transport of DsRed-labelled mitochondria in rat cortical neurons transfected with acGFP1-tagged LRRK2 variants as indicated and treated with vehicle (Ctrl) or 0.5 μM TSA for 4 h. Charts are mean±s.e.m. of quantified mitochondrial transport shown as percentage of total mitochondria. (b) Kymographs of mitochondria in transgenic Drosophila motor neurons expressing mito-GFP and indicated LRRK2 variants. Ctrl is expressing a lacZ transgene. Drosophila were raised on food containing vehicle control (Ctrl) or 10 μM TSA. Charts are mean±s.e.m. of quantified mitochondrial transport shown as percentage of total mitochondria. (c) Locomotion assays for climbing behavior following vehicle control or TSA treatment. Drosophila were raised on normal food, then fed vehicle (Ctrl) or TSA supplemented food for 5 days before testing. Transgene expression is driven by D42-GAL4. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, two-way analysis of variance followed by Fisher’s least significant difference post hoc test. (a) N (cells; from three to five experiments)=WT Ctrl, 30; WT TSA, 7; Y1699C Ctrl, 20; Y1699C TSA, 5; (b) N (animals)=WT Ctrl, 10; WT+TSA, 10; R1441C Ctrl, 13; R1441C+TSA, 13; Y1699C Ctrl, 11; Y1699C+TSA, 11; (c), right chart: N (animals)=Ctrl Ctrl, 84; Ctrl+TSA, 77; WT Ctrl, 68; WT+TSA, 46; R1441C Ctrl, 43; R1441C+TSA, 53; left chart: N (animals)=Ctrl Ctrl, 41; Ctrl+TSA, 45; WT Ctrl, 56; WT+TSA, 78; Y1699C Ctrl, 59; Y1699C+TSA, 87.