Figure 8: AUF1 protects cells from DNA damage.
From: PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity
(a) AUF1 and HuR target mRNAs downregulated (as determined by RNA-Seq) in senescent (S) WI-38 human diploid fibroblasts. (b) Relative expression (as assessed by RNA-Seq) of target mRNAs in proliferating and senescent fibroblasts, as well as in proliferating WI-38 cells after transfection of control, AUF1 or HuR siRNAs. (c) Schematic of AUF1- and HuR-binding sites on HNRNPK and H3F3A mRNAs. (d,e) Cells prepared as described in b were used for assessment of steady-state levels using RT-qPCR (d) and relative stability (e) of HNRNPK and H3F3A mRNAs; mRNA levels and half-lives were calculated as described in Fig. 3b; data are the means and s.d. from three independent experiments. (f,g) DNA fragmentation assay (f) and comet assay (g) were performed after transfection of HeLa cells with control (Ctrl) siRNA or AUF1 siRNA. *** P<0.001.
