Figure 2: First phase insulin release is blunted when basal bodies/cilia are disrupted. | Nature Communications

Figure 2: First phase insulin release is blunted when basal bodies/cilia are disrupted.

From: Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents

Figure 2

(a) Whole islets depleted of Ofd1(blue) or Bbs4 (red) have an attenuated first phase when stimulated with glucose but react normally to potassium chloride (t=58 min, **P=0.001 (one-way ANOVA), error bars: st. dev.). (b) Re-introduction of myc-tagged Bbs4 rescues the disposition index in shBbs4-treated cells. P(shNC versus shBbs4)=0.024; P(shBbs4 versus shBbs4+1,600 ng mycBBS4)=0.000; P(shNC versus shBbs4+1,600 ng mycBBS4)=0.994 (one-way ANOVA). (c) The acute insulin response to glucose in Bbs4−/− mice (red) is significantly attenuated compared with age- and weight-matched controls (black; n=4, t=5 min, *P=0.039, error bars: s.e.m.).

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