Figure 4: Scanner view of the spots.

(a) Typical scanner section of a pipette spot at 3 nM (left) and of a PC spot at 300 aM (right), scale bar, 200 μm. (b) Typical scanner sections containing the images of four spots corresponding to most challenging dilutions (from left to right: 300 fM, 30 fM, 3 fM, 300 aM) in case of (I) pipette and (II) PC, scale bar, 800 μm.