Figure 1: Transcriptional directionality and exosome sensitivity of RNAs emitted from DHSs.
From: Nuclear stability and transcriptional directionality separate functionally distinct RNA species
(a) Average exosome sensitivity (vertical axis) of RNAs emanating from transcribed DHSs, broken up by strand as well as by transcriptional direction bias in control HeLa cell CAGE (indicated by colour coding). Exosome sensitivity was measured as the ratio between CAGE expressions from hRRP40-depleted versus control HeLa cells and plotted relative to DHS summits (horizontal axis). (b) Average HeLa GRO-seq signal (per 10 bp) in a 1-kb window centred at DHS summits broken up as above. (c) Average ENCODE27 HeLa ChIP signal of the RNAPII subunit POLR2A (left panel) or RNAPII with the Ser2 carboxy-terminal domain phosphorylated (right panel) in 1 kb regions centred at DHS summits, broken up as above. (d) Fraction (vertical axis) of bidirectional and unidirectional DHSs (broken up by strand) as determined by GRO-seq data, with TATA box motif hits25 on indicated strand (horizontal axis). Average profiles in a–c have been smoothed.
