Figure 3: Lbx2 does not regulate the subcellular localization of β-catenin.
From: Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway
(a–f) Immunolocalization of β-catenin on (a–c) cryosections and (d–f) whole-mount mid-gastrula embryos. (a) WT, (b) Wnt8a morphant, (c) Lbx2 morphant. Lower panels in a–c are close-up views of the rectangular regions of the embryos in the upper row with left: β-catenin (green), centre: nuclei labelled with DAPI (blue) and right: merge of the two channels. This boxed domain corresponds to the posterior ventral domain where Lbx2 is expressed in the mesoderm (indicated with red dotted lines). (d–f) Lateral views of embryos, (d) WT, (e,f) injected with (e) 25 pg Wnt8a mRNA or (f) 40 pg Lbx2 mRNA showing that, whereas (e) gain of function of Wnt8a results in an accumulation of β-catenin into the nuclei of all cells of the gastrula, (f) the nuclear accumulation of β-catenin for an embryo overexpressing Lbx2 RNA is similar to (d) WT, with the animal pole devoid of nuclear β-catenin. Bottom row: close-up of the animal pole region in animal pole view for the different conditions used. Scale bar in a is equivalent to: 80 μm (a–c top), 20 μm (a–c bottom), 100 μm (d–f top) and 10 μm (d–f bottom).
