Figure 1: Nitrate uptake and reduction in plants with altered NO signalling. | Nature Communications

Figure 1: Nitrate uptake and reduction in plants with altered NO signalling.

From: S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Figure 1

(a) Expression of the NRT marker genes NRT1.1 and NRT2.1 in the roots of WT, nox1 and gsnor1 plants was determined by quantitative reverse transcriptase-PCR (qRT–PCR) and normalized to expression of ACT2. Error bars represent s.d. (n=3). (b) Effect of GSNO on nitrate-induced expression of NRT genes in roots. WT seedlings grown in half-strength MS medium (9.4 mM KNO3 and 10.3 mM NH4NO3) were incubated for 3 h in water with 1 mM nitrate (KNO3), in the absence or presence of GSNO or DEA/NO. NRT expression was determined by qRT–PCR and normalized to expression of ACT2. Error bars represent s.d. (n=3). (c) NR activity and (d) nitrate (NO3−) content determined in leaf extracts of WT plants and genotypes with enhanced (nox1 and gsnor1) or impaired (nia1 nia2 and 35S::FLAG-GSNOR1) (S)NO homeostasis, after 6 hours of light. Data points represent means±s.d. of three independent experiments. Asterisks indicate statistical differences from the WT (Student’s t-test, P<0.05).

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