Figure 3: Nitrate-derived NO suppresses activity of GSNOR1. | Nature Communications

Figure 3: Nitrate-derived NO suppresses activity of GSNOR1.

From: S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Figure 3

(a) GSNOR activity, (b) NR activity, (c) NO emission and (d) GSNOR1 gene expression measured in leaf extracts of WT and nia1 nia2 plants grown under low or high nitrate availability. Plants were grown in perlite:vermiculite (1:1) under a 12/12 h light/dark period and irrigated three times a week with MS nutrient solution containing 2.5 or 25 mM nitrate (half KNO3 and half NH4NO3). All measurements were taken 6 hours post light exposure. Relative expression of GSNOR1 in (d) was determined by quantitative reverse transcriptase-PCR and normalized to expression of ACT2. (e) GSNOR activity in genotypes with impaired and enhanced (S) NO signalling. Plants were grown in soil with a photoperiod of 16/8 h light/dark and irrigated with water as needed. Data points represent means±s.d. of three independent experiments. Asterisks indicate significant differences from the WT (Student’s t-test, P<0.05).

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