Figure 4: NO inhibits GSNOR1 by S-nitrosylation. | Nature Communications

Figure 4: NO inhibits GSNOR1 by S-nitrosylation.

From: S-nitrosothiols regulate nitric oxide production and storage in plants through the nitrogen assimilation pathway

Figure 4

(a) Dose response of GSNOR activity in WT leaf extracts supplemented with the S-nitrosylating agents DEA/NO and Cys-NO or the redox-active molecules GSH and L-Cys. (b) GSNOR activity in WT leaf extracts supplemented with indicated intermediates of the nitrogen assimilation pathway. (c) GSNOR activity in leaf extracts of WT plants fumigated with NO gas (60 p.p.m.) or with normal air for 12 h. (d) Cys-NO induced S-nitrosylation of GSNOR1 in vitro. Leaf extracts from 35S::FLAG-GSNOR1 plants in WT background were exposed to Cys-NO and subjected to the biotin switch technique. Total GSNOR1 protein ensures equal protein loading. The position of a 50-kDa marker is indicated. (e) In vivo S-nitrosylation of GSNOR1 using a 35S::FLAG-GSNOR1 construct in WT and nox1 background. SNO-GSNOR1 was analysed and detected as in d. The position of a 55-kDa marker is indicated. Data points from a to c represent means±s.d. of at least three independent experiments. Asterisks indicate significant differences from the controls (Student’s t-test, P<0.05).

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