Figure 2: BRMS1L suppresses breast cancer migration and invasion.
From: BRMS1L suppresses breast cancer metastasis by inducing epigenetic silence of FZD10
(a) Invasion of cancer cells measured by Boyden chamber assays in BT-474 cells that were mock transfected (mock), or transfected with GFP-siRNA or either of the two BRMS1L-siRNAs (BRL-si1 and BRL-si2) and in MDA-MB-231 cells transfected with a pcDNA3 vector carrying BRMS1L (BRMS1L) or an empty vector as negative control (NC). Scale bars correspond to 50 μm. Bars correspond to mean±s.d. ***P≪0.001 as compared with GFP-siRNA. ###P≪0.001, as compared with NC. Student’s t-test was used for the comparison of two independent groups. (b) Wound-healing assay for MDA-MB-231 cells that were mock transfected (mock), or transfected with GFP-siRNA, BRMS1L-siRNAs, pcDNA3 vector carrying BRMS1L (BRMS1L) or negative control (NC). Bars correspond to mean±s.d. **P≪0.01 as compared with GFP-siRNA. ##P≪0.01, as compared with NC. Scale bar corresponds to 200 μm. Student’s t-test was used for the comparison of two independent groups. (c–e) Phase-contrast microscopy (c), immunofluorescent staining (d) and western blotting (e) for MCF-10A that were mock transfected, or transfected with GFP-siRNA or BRMS1L-siRNAs. Scale bars correspond to 50 μm. BRL, BRMS1L.
