Figure 5: BRMS1L suppresses WNT3/FZD10/β-catenin pathway.

(a) Wnt/β-catenin activity measured by TCF/LEF luciferase reporter assays in T47D cells that were mock transfected (mock), or transfected with GFP-siRNA or two BRMS1L-siRNAs and in MDA-MB-231 cells that were transfected with pcDNA3 vector carrying BRMS1L (BRMS1L) or negative control (NC). Bars correspond to mean±s.d., ***P≪0.001 as compared with GFP-siRNA, ^###P≪0.001 as compared with NC. (b,c) Wnt/β-catenin activities measured by TCF/LEF luciferase assays in MDA-MB-231 (b) and T47D cells (c) treated as indicated. Bars correspond to mean±s.d. **P≪0.001 as compared with BRMS1L or BRMS1L-siRNA1. (d,e) Western blotting for nuclear (n) and cytoplasm (c) β-catenin in MDA-MB-231 (d) and T47D cells (e) treated as indicated. (f,g) Wnt/β-catenin activities measured by TCF/LEF luciferase assays (f) and β-catenin translocation (g) in MDA-MB-231cells treated as indicated. ***P≪0.001 as compared with GFP-siRNA. (h,i) Wnt/β-catenin activities measured by TCF/LEF β-catenin translocation (h) and luciferase assays (i) in T47D cells treated as indicated. ***P≪0.001 as compared with BRMS1L-siRNA1. β-cat, β-catenin; BRL, BRMS1L. Bars correspond to mean±s.d. These data are representative of three independent experiments. Student’s t-test was used for the comparison of two independent groups in a,b,c,f,i.