Figure 6: Increasing ADP availability by apyrase application amplifies astrocytic hyperactivity and calcium waves. | Nature Communications

Figure 6: Increasing ADP availability by apyrase application amplifies astrocytic hyperactivity and calcium waves.

From: Metabotropic P2Y1 receptor signalling mediates astrocytic hyperactivity in vivo in an Alzheimer’s disease mouse model

Figure 6

(a) Augmenting the hydrolysis of ATP to ADP, while minimizing ADP degradation, using the ectonuleotidase apyrase significantly increased the frequency of astrocytic transients in all active astrocytes (P<0.05, Mann–Whitney test; n=126 astrocytes from n=3 mice). (b) Apyrase significantly increased the fraction of hyperactive astrocytes and decreased the fraction of inactive astrocytes in APPPS1 mice (P<0.05, χ2-test). (c) Apyrase significantly increase the frequency of astrocytic calcium waves in APPPS1 mice (P<0.05, Mann–Whitney test). (d,e) There was a nonsignificant trend towards a higher velocity of calcium waves after apyrase treatment. No significant difference was observed in the maximal distance calcium waves propagated within the imaging plane (P>0.05, Mann–Whitney test for both).

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