Figure 5: Synergistic upregulation of pro-inflammatory cytokines on c-IgG-TLR ligand co-stimulation is dependent on FcγRs and Syk. | Nature Communications

Figure 5: Synergistic upregulation of pro-inflammatory cytokines on c-IgG-TLR ligand co-stimulation is dependent on FcγRs and Syk.

From: Fc gamma receptor-TLR cross-talk elicits pro-inflammatory cytokine production by human M2 macrophages

Figure 5

(a) FcγR expression (10log fluorescence intensity) on unstimulated M2 macrophages was analysed by flow cytometry. (b) Before stimulation, M2 macrophages were incubated with blocking antibodies against indicated FcγRs or (c) 1 μM of Syk inhibitor R406. (a–c) Data are representative of at least three experiments with different donors. (b,c) After 24 h, cytokine levels were determined by ELISA, mean+s.e.m. Experiments were performed in triplicate.

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