Figure 1: BBR reduces obesity and improves insulin sensitivity.

(a) Body weight evaluation of db/db mice treated with vehicle or BBR every 3 days (n=5). (b) Fat mass expressed as percentage of total body weight-which is shown as ratio of subcutaneous fat to body weight. (n=5). (c) Free fatty acid (FFA) levels were measured after overnight fast in the serum of mice treated with vehicle or BBR (n=5). (d) i.p. glucose tolerance test on db/db mice treated as in a and injected with 0.75 g glucose per kg after overnight fast (n=5). (e) The adjacent bar graph represents the average area under the curve (n=5). (f) Serum insulin concentration at 0 min (fasted overnight) and after glucose load at 30 and 60 min during i.p. glucose tolerance test (n=5). (g) Insulin tolerance test was evaluated throughout and injected with 1 U insulin per kg after 6 h of fasting (n=8). (h) Rectal temperature measured at 0900 hours (n=7–9). Values represent mean±s.e.m. Error bars represent s.e.m., and significant differences compared with vehicle controls are indicated by *P≪0.05, **P≪0.01 and ***P≪0.001 (assessed by Student's t-test).