Figure 3: SCAR-dependent lamellipodia promote egg chamber rotation. | Nature Communications

Figure 3: SCAR-dependent lamellipodia promote egg chamber rotation.

From: Epithelial rotation promotes the global alignment of contractile actin bundles during Drosophila egg chamber elongation

Figure 3

(a) Live imaging was used to determine the contributions of lamellipodia (Abi) and filopodia (Ena) to follicle cell (FC) migration. Expressing Abi RNAi in all follicle cells throughout oogenesis with the traffic jam-Gal4 (tj-Gal4) driver blocks the rotation of stage seven–eight egg chambers, whereas ena RNAi has no effect. Individual data points, mean±s.e.m., t-test: ***P=1.7 × 10−7. (b) Abi RNAi egg chambers fail to elongate properly beginning at stage six, whereas ena RNAi does not disrupt elongation. The aspect ratio is the length of the egg chamber divided by its width. For stages 4–10 n≥6, stages 11–13 n≥3, stage 14 n≥25 (exact n values are in Supplementary Table 2). Data represent mean±s.d., t-test: **P<0.003, ***P<0.0008. (c) The basement membrane fails to become polarized when rotation is blocked by Abi depletion. Basement membrane structure is visualized using a GFP protein trap in the Collagen IV (Col IV) α2 chain, Viking (Vkg GFP). (d) Expression of Abi RNAi with tj-Gal4 eliminates leading edge structures and disrupts the tissue-level alignment of the basal actin bundles. (c,d) Laser-scanning confocal images of stage seven-eight egg chambers. Scale bars, 10 μm.

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