Figure 4: Egg chamber rotation begins at stage one.

(a) SCAR is enriched at one side of each follicle cell during early rotation stages. Scanning confocal image of the basal epithelial surface of a stage two egg chamber. (b) Stills from a time series of His2Av RFP-expressing egg chambers that show rotation occurring at stages one (0.10 μm min−1), three (0.34 μm min−1) and four (0.32 μm min−1). The coloured dots mark the same set of nuclei through each 30-min interval. Spinning disk confocal images. (c) Quantification of follicle cell (FC) migration rate. Each data point represents and individual egg chamber with mean±s.d. Scale bars, 10 μm.