Figure 2: Molecular sieving characteristics of pOEGMA–ASNase conjugates.
From: Semi-permeable coatings fabricated from comb-polymers efficiently protect proteins in vivo
(a) Representative 1H NMR spectrum of a pOEGMA–ASNase conjugate with peaks corresponding to ‘A’ and ‘B’ from Fig. 1a identified. (b) Analysis of the flexibility F of the pOEGMA backbone permits the identification of the transition between ellipsoidal to cylindrical at the local minimum of the curve (arrow). (c,d) Enzymatic activity and anti-ASNase-binding affinity of ASNase–polymer conjugates, both relative to native ASNase, plotted as a function of polymer backbone length n. In c, star symbols indicate that the measured activity is statistically different from the value at lowest n (ANOVA, Tukey P=0.05; Supplementary Table 2). The sigmoidal curve fit (c) and single exponential decay (d) used to fit the data are to guide the eye. mPEG–ASNase is shown as a line and a symbol in the centre of the graph for ease of comparison. Mean±s.d. (n=3).
