Figure 3: Upregulated miRNAs in the odour-conditioned bees and the predicted mRNA–miRNA-target network.
From: Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee
(a) qRT-PCR validation of seven miRNAs upregulated in odour-conditioned bees represented as a fold change in gene expression compared with the control group (air) using 18S rRNA as an endogenous control gene. Expression levels shown are means of three technical replicates for each sample. (b) Composite miRNA and PPI network conserved in honeybee and fruit fly. Green nodes represent target genes downregulated in odour-conditioned bees, and grey nodes represent selected miRNAs upregulated in odour-conditioned bees including miR-210 and miR-932 (red nodes) that we functionally characterized. Node labels are gene name or accession number (Beebase, Flybase and miRBase). Thick edges depict interactions between proteins and arrows depict predicted miRNA-binding sites. The size of the green nodes is proportional to their level of differential expression (Supplementary Data 1).
