Figure 5: Functional roles of miR-932 and miR-210 in learning and memory.
From: Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee
(a) Learning curves for six odour-conditioning trials with linalool, showing percentage of correct proboscis extension response (PER), comparing bees 24 h after treatment with either sugar (white, n=32), scrambled antisense miRNA (grey, n=30), antisense miR-932 antagomir (red, n=38) or antisense miR-210 antagomir (green, n=44). (b) Memory recall in same groups of bees 48 h after treatment, showing percentage of correct PER; numbers in bars are numbers of bees per groups; different letters above bars indicate significant difference at P<0.05 (GLM). (c) Relative expression of miR-932 (light grey) and miR-210 (dark grey) in bee brains determined with qRT-PCR, 24 and 48 h after treatment with a-miR-932 and a-miR-210, respectively, compared with expression in brains treated with scrambled antisense miRNA as control. Sugar treatment was used as a calibrator group for calculating ΔΔCT. Expression levels shown are means±s.d. of three biological replicates compared with scrambled as baseline (dashed line). Statistical analysis was performed using Welch’s t-test (*P<0.01).
