Figure 6: Dual-luciferase reporter analysis of miR-932 target interactions.
From: Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee
Shown are changes in expression ratio of Firefly (Fluc) and chimeric Renilla luciferase (Rluc) containing putative 3′UTR and miRNA regulatory elements (MREs) of target genes treated with and without (±) exogenous miR-932 mimic and scrambled RNA. Welch’s two-sample t-test was used to verify MRE and 3′UTR target associations, t-test *P<0.01. The 3′UTR of Act5C (a: Ψ-Act5C_3UTR; t=4.35, df=6.55, P<0.01), positive control, perfect match 23-bp miR-932 ‘seed’ sequence (d: Ψ-positive_ctrl; t=6.41, df=5.93, P<0.01) and the native MRE identified in the 3′UTR of Act5C (e: Ψ-Act5C_MRE_wt; t=5.24, df=3.671, P<0.01) are shown to be significantly downregulated by miR-932. The 3′UTR of Sec61b (b) and His1 (c) were not targeted by the miR-932 mimic. Similarly, the mutant control (f: Ψ-Act5C_MRE_mut) was not downregulated by miR-932.
