Figure 6: Disruption of the SH3–GUK interactions abrogates clustering and NF-kB activation by oncogenic CARMA1. | Nature Communications

Figure 6: Disruption of the SH3–GUK interactions abrogates clustering and NF-kB activation by oncogenic CARMA1.

From: Clustering of CARMA1 through SH3–GUK domain interactions is required for its activation of NF-κB signalling

Figure 6

(a,b) Jurkat T cells expressing WT, L251P, L251PV801P or L251PL815P CARMA1-mEGFP (green) were imaged by a fluorescence microscopy (a). Representative images of single cells at a higher magnification are shown in the insets. The arrowheads indicate clusters of CARMA1. Scale bars, 5 μm. The percentage of cells forming CARMA1 clusters is shown in b (n=200). (c) JPM50.6 cells were transfected with plasmids encoding an NF-κB luciferase reporter plasmid (Firefly luciferase) together with expression plasmids encoding mock, WT, L251P, L251PV801P or L251PL815P CARMA1. Twenty-four hours after transfection, cells were left unstimulated (–) or stimulated with PMA (10 ng ml−1) plus calcium ionophore (1 μM; P/I) for 6 h. Cells were lysed and luciferase activity was determined. All samples were also transfected with constitutively expressed Renilla luciferase to normalize the transfection efficiency. A representative of three independent experiments is shown.

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