Figure 4: Reconstructed images of actin filaments in a live BSC-1 cell stained with PA-RhB-labelled probe 3. | Nature Communications

Figure 4: Reconstructed images of actin filaments in a live BSC-1 cell stained with PA-RhB-labelled probe 3.

From: A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging

Figure 4

(a) Diffraction-limited TIRF (upper right) and super-resolution (lower left) images. (b) Enlarged super-resolution image of the blue-boxed region in a and cross-sectional profiles of the actin filaments with full-width at half-maximum (FWHM) of 83 nm. (c) Enlarged super-resolution time-lapse images of the yellow-boxed region in a. Each image was reconstructed from 1,000 frames at 100 Hz. (d) Enlarged image of the boxed region in c at 40 s. Scale bars, (a) 5 μm, (b) 200 nm and (c) 500 nm.

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