Figure 5: Reconstructed images of lysosomes in a live HeLa cell stained with Caged-Rh110-labelled probe 5. | Nature Communications

Figure 5: Reconstructed images of lysosomes in a live HeLa cell stained with Caged-Rh110-labelled probe 5.

From: A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging

Figure 5

(a) Diffraction-limited TIRF. (b) Super-resolution image. (c) Enlarged TIRF image of the boxed region in a. (d) Enlarged super-resolution image of the boxed region in b. Super-resolution image was reconstructed from 500 frames at 260 Hz. (e) Cross-sectional profiles of lysosome shown in c,d (diffraction-limited, blue line; super-resolved, red line with a full-width at half-maximum (FWHM) of 64 nm). Scale bars, (b) 2 μm and (d) 200 nm.

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